Bifidobacterium longum subsp. longum BBMN68 was a probiotic separated from intestine in centenarians in Bama， Guangxi， China. The article used bioinformatics technique to analyze the oligosaccharide glycose hydrolase genes， and validated the usage of relevant oligosaccharides via growth experiment and RT-PCR. Results showed that BBMN68 had various glycoside hydrolases， which may potentially degrade oligosaccharides into monosaccharides including galactose， glucose， N-acetylglucosamine， N-acetylgalactosamine， fructose， mannose， arabinose and xylose. BBMN68 can grow in a variety of oligosaccharide mediums including XOS， GOS， IMO， D-maltose and FOS. Oligosaccharide transporter genes（BBMN68_74， BBMN68_75， BBMN68_217， BBMN68_925， etc.） were induced to express via RT-PCR in different experimental conditions.