The in vitro simulation intestinal microecology system is available method of easy-opeartion and good-reproducibility， which can be used to study intestinal microbiota and its metabolites gradually. In this study， the beneficial prebiotic effects of Luffa cylindrica， Morus alba， Lycium barbarum and Lentinus edodes freeze-dried powders were studied using a system of in vitrosimulation intestinal microecology. The results showed that Luffa cylindrica significantly increased the more numbers of Lactobacillus， Prevotella， Bacteroides and higherpropionate acidcontent with in vitro fermentationfor 24 hours， which was better than other vegetable and fruit powders did. Meanwhile， Morus alba promoted a largerincrease （P<0.05） of the number of Bifidobacterium and the production of acetate acid than others did. These findings suggest that Luffa cylindrica and Morus alba probably display betterprebiotic effects on intestinal microbiotaon the basis of in vitro simulation intestinal microecology system.