The current normal method for the determination of benzo（a）pyrene is not specific， and its sensitivity is not very good. The 3，4-benzo（a）pyrene（3，4-BaP） is an organic compound with the formula C20H12. 3，4-BaP is a harmful compound， because it generates carcinogenic and mutagenic metabolites which can intercalate into DNA and interference with transcription. In this study， the molecular imprinted sorbent was synthesized and packed into a solid-phase extraction （SPE） cartridge for the enrichment of 3，4-BaP in smoked sturgeon （Acipenser sinensis） bought from local market. An analytical method was also developed for accurate determination of 3，4-BaP on the basis of ultra-performance liquid chromatography tandem mass spectrometry. The molecular imprinted sorbents are dispersed spherical particles， with diameter from 20 to 90 μm. It was successfully applied in the enriching of 3，4-BaP， and its performance was compared with Sep-pak， HLB， and Florisil SPE. The method was validated， and the results showed that the calibration curve is linear with R2 = 0.9998. The limit of detection （LoD） and limit of quantitation （LoQ） are 0.98 and 3.16 μg/kg， respectively. The RSDs of intra-day and inter-day accuracy are 3.26%-4.77% and 3.78%-5.26%， respectively. The recovery ranged from 89.7% to 95.3%， with RSD 2.89%-3.44%. Afterwards， the method was successfully applied to determine the 3，4-BaP in 50 batches of smoked sturgeon. The results indicated that 12 positive samples were found. The content of 3，4-BaP in the worst sample reached 21.9 μg/kg. The contents of 3，4-BaP in the other samples are lower than 3.58 μg/kg. In conclusion， this method is good at determining 3，4-BaP in smoked sturgeon. The relevant data could be used as reference for the functional departments for ensuring the food safety for consumers.