Isolation and Identification of Beneficial Bacteria in Bohai Shrimp Paste
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    Abstract:

    Shrimp paste, one of the Chinese traditional digesters, can promote the growth of anaerobic bacteria in intestines and balance the intestinal flora, which is beneficial to the intestinal digestion and absorption. Based on the Bohai shrimp paste as the research object, we isolated a strain of digestive bacteria in the shrimp paste with the nutrient agar medium, and found it positive by gram staining, 16S rDNA amplification and sequencing, BLAST alignment in NCBI databases and build strain phylogenetic tree showed it belonged to Bacillus licheniformis, which was consistent with the above characteristics. Heat resistance analysis of the strain found that there was no significant decrease on its survival rate at less than 40 ℃, and the survival rate was gradually decreased with temperature increasing, which indicated that the heat treatment in shrimp paste processing would attenuate its digestive activity. This study will provide a theoretical foundation for the digestive activity preservation of Bohai shrimp paste in food heating process.

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  • Online: April 14,2020
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