This study designed two pairs of primers and probes and established a duplex digital PCR method for the quantitative detection of GM rice TT51-1 event based on the sequence of sucrose phosphate synthase gene of rice and TT51-1 event-specific gene， respectively. The absolute sensitivity and relative sensitivity were 2 copies/μL and 0.1% respectively. The relative standard deviation （RSD） was among 7.30% to 18.63% while the deviation was among - 8.77% to 9.62% in 6 replicates， even the content of GM rice TT51-1 event was as low as 0.1%， met the requirements of standardized detection of GMO. The relative sensitivity of real-time PCR method established with the same pairs of primers and probes could reach only 1%. In order to promote the standardization the application of this method， an intra-laboratory repeatability validation was performed on the chip-based digital PCR platform by different operators to verify the quantitative detection method established on the droplet digital PCR platform， and the results showed that the precision and accuracy of the assay still met the quantitative requirements. It was proved that this method could be applied to the quantitative detection of TT51-1 GM rice ingredients in rice and its preliminary processing products.