A simple， efficienct， sensitive method for the determination of phthalates（PAEs） in seafood using GC-MS was proposed. Moreover， the contents of PAEs in different seafoods were determined， and their health risk assessment was evaluated， simultaneously. Under optimized condition， the linearity of this method and detection limits （3S/N） were in the range of 1-1 000 μg/L and 0.15-0.30 μg/kg， respectively. The recoveries of spiked 16 PAEs at low， medium and high three gradient levels were in the ranges of 73%-117%， with relative standard deviations of 2.8%-7.5% （n=5）. The results showed that there was no significant difference in contents of PAEs among different type of seafood， and the∑PAEs ranged from 80.0 μg/kg to 438 μg/kg. diisobutyl phthalate （DIBP）， di-n-butyl phthalate （DBP） and di-2-Ethylhexyl phthalate （DEHP） were the predominant compounds in all detected seafood， accounts for 83.01%-94.18% of the total PAEs. Based on health risk assessment， all detected seafoods were considered safe for consumption， and children’s health risk was slightly higher than adults.