Antibiotics resistance of 97 lactic acid bacteria isolated from traditional fermented foods were analyzed. The results showed that most of strains were resistant to vancomycin， gentamicin， kanamycin， streptomycin， tetracycline， and teicoplanin， and the resistance rate could reach more than 50%. However， the resistance to ampicillin， erythromycin， trimethoprim and cefuroxime were relatively lower， just about 10%. Among of the 13 antimicrobial resistance genes， the most detected genes were tetM（91.75%） and ermB（86.60%）， followed by aac（6′）-aph（2″）（53.61%）， lnuA（45.36%）， tetK（39.18%）， ermC（36.08%）， strA（34.02%） and blaZ（32.99%） genes， and strA（27.84%）， msrA/B（22.22%） and aph（3′）-III（7.22%） was lower detected genes. The cat and vanX gene were not detected in our study. Additionally， by MTP methods， 70% of isolates were determined to have biofilm producing ability. Compared to the biofilm-producing negative strains， the positive ones were proved to be more frequent multiple resistant. In conclusion， the transmission of multiple drug resistance（MDR） bacteria and related genes would been a huge risk to food safety. Bull antibiotic restrictions policy and programs should be promoted to reduce the selection pressure of antibiotics， and the safety evaluation of the edible strains should been strengthened.