Lactoferrin is a unique ingredient in milk, which has the functions of broad-spectrum antibacterial, anti-inflammatory， inhibition of tumor cell growth and regulation of immune response. It is a new antimicrobial and anticancer component. In this experiment， the new antimicrobial peptide FP-PF was designed with the idea of a symmetrical structure as the basis， and melittin was used as the positive control peptide. The cytotoxicity， hemolytic activity and bacteriostatic mechanism of the new antimicrobial peptides FP-PF were studied. The experimental results showed that the hemolysis rate of FP-PF which concentation was at the range of 1-256 mmol/L was less than 5%， indicating that the hemolysis rate was low. The cell survival rate was more than 92%， indicating that its cytotoxicity was small. Through the study of bacteriostatic mechanism， the antibacterial peptide FP-PF could damage the outer and inner membranes of cell membranes， change its osmotic pressure and integrity， and thus achieve the effect of sterilization and bacteriostasis. Therefore， the new antimicrobial peptide FP-PF designed in this experiment was expected to show its potential application value in food， cosmetics and so on.