Yarrowia lipolytica Y-2 screened by our laboratory is a strain with the ability of degrading OTA in vitro. The main purposes of this study was to investigate the control of postharvest disease of different varieties of grapes caused by Talaromyces rugulosus O1 and OTA accumulation by Y. lipolytica Y-2， the control of postharvest disease of Red Globe grapes and OTA accumulation by Y. lipolytica Y-2 at different concentrations， as well as the control of postharvest disease of Red Globe grapes and OTA accumulation during the entire storage period. The results indicated that Y. lipolytica Y-2 showed significant biocontrol activity for postharvest disease of different varieties of grapes and OTA accumulation. Compared with the control， the lesion diameters of Summer grapes， Huge Peak grapes and Red Globe grapes treated with Y. lipolytica Y-2 were decreased by 28.7%， 19.7% and 18.5%， respectively， and OTA accumulation in the wound of Huge Peak grapes was decreased by 83.3%. The biocontrol efficacy was enhanced as the increase of the concentration of Y. lipolytica Y-2. In comparison with the control， the lesion diameter of Red Globe grapes treated with Y. lipolytica Y-2 at concentration of 1 × 108 cells/mL decreased by 23.8%， while OTA accumulation decreased from 50 ng/wound to 5.95 ng/wound. Y. lipolytica Y-2 showed significant biocontrol activity for the postharvest disease of Red Globe grapes and OTA accumulation during the entire storage period.