AHLs are a kind of signal molecules that mediate quorum sensing of gram-negative bacteria. Screening microorganisms with degradative activity on AHLs from nature as a quorum sensing inhibitor is a new strategy for controlling pathogenic bacteria. 56 strains of degrading Aeromonas hydrophila quorum-sensing AHLs signaling molecules were rapidly screened out from 156 strains of lactic acid bacteria that isolated from traditional fermented foods and marine fish intestines using a 96-well plate method. The initial screening rate was 35.90%. 56 strains of lactic acid bacteria screened out 12 strains with strong degradation activity by agar diffusion method. The degradation activity of strain YP 4-1-1 derived from the intestine of marine fish gingiva was close to 100%. Analysis by GC-MS showed that the activity of the strain YP 4-1-1 degrading Aeromonas hydrophila C4-HSL and C6-HSL was 98.31% and 81.06%， respectively. The physiological and biochemical reactions and 16S rRNA sequence analysis confirmed that strain YP 4-1-1 was Lactobacillus sakei. Further studies showed that the quorum quenching enzyme activity of strain YP 4-1-1 is present in the soluble fraction of the crude cell extract， and may be an AHLs-acyltransferase or oxidoreductase. The MICs of YP 4-1-1 crude extracts against Chromobacterium violaceum CV026 and Aeromonas hydrophila were 2.0 mg/mL and 4.0 mg/mL， and three different sub-MICs（1/4 MIC， 1/2 MIC and 3/4 MIC） dose-dependent degradation of the purple pigment. In vitro co-culture experiments， strain YP 4-1-1 attenuated the expression of virulence factors such as hemolysin， DNase and lipase of Aeromonas hydrophila， and had inhibitory activity on quorum-sensing clustering and migration. In this paper， strains with degrading activity against gram-negative bacteria AHLs signal molecules are obtained from lactic acid bacteria in the natural environment， which lays the theoretical and application foundation for the development of lactic acid bacteria biological agents that control quorum sensing.