Objective： This article was designed to study the antioxidant activities of two kinds of stevia residue extracts on D-galactose（D-gal） induced aging mice. Methods： Eighty mice were randomly divided into control group， D-gal group， low， medium and high dose of two kinds of stevia residue extracts（100， 200， 500 mg/kg bw） groups. The aging model was established by subcutaneous injection with D-gal（500 mg/kg bw）， and the mice in the control group were injected subcutaneously with the same volume of normal saline. The mice in the sample groups were given different doses of stevia residue extracts by gavage. After 11 weeks， the activity of superoxide dismutase （SOD）， glutathione peroxidase （GPx）， and the content of malondialdehyde （MDA） in serum， liver and brain， and the relative expression of antioxidant enzyme gene in brain tissues were measured. Results： Compared with D-gal group， two kinds of stevia residue extracts could increase the activity of serum， liver and brain antioxidant enzymes （SOD and GPx） and decrease the content of MDA in different degree， and the effect was the most obvious at high dose （500 mg/kg bw）. The relative expression of antioxidant enzyme genes （Nrf-2， SOD1， GPx1， HO-1） in brain tissue of mice in the high dose group （500 mg/kg bw） was significantly increased. Conclusion： The two kinds of stevia residue extracts can increase the activity of antioxidant enzymes and decrease the MDA level in mice by promoting the gene expression level of antioxidant enzymes and possess potential anti-aging effect.