It is aimed to investigate the applicability for identifying Staphylococcus argenteus by GB 4789.10-2016 method， and then to establish a complementary method. Total 193 isolates of Staphylococcus aureus and 6 isolates of Staphylococcus argenteus were used in the identification with the both methods either recommended in GB 4789.10-2016 or PCR amplification of nuc1 and NRPS genes. And according to the results of biochemical characteristics and PCR amplification， it was unfortunate that S. argenteus strains were all falsely detected as S. aureus with the method recommended in GB 4789.10-2016 and the nuc1-PCR method， however， the NRPS-PCR method established in this study can distinguish these two species. Therefore， a new protocol was established based on the combination of GB 4789.10-2016 and NRPS-PCR to identify and distinguish S. aureus and S. argenteus.