Prevalence and Serogroup of Listeria monocytogenes in Agri-food
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    Abstract:

    Listeria monocytogenes is an important foodborne pathogen with high mortality rates. A total of 437 samples with 4 kinds of agri-food (fresh meat, egg, milk and vegetable) were collected from supermarkets, farmers markets and production base in Shanghai. L. monocytogens were isolated from these samples by GB 4789.30-2010 standard method, and the isolates serogroup was identified by multiple PCR. Results showed that total detection rate of L. monocytogenes was 14.9% (65/437). The percentage distribution of the isolates was 27.2% (61/224), 3.4% (2/58), 2.0% (1/51) and 1.0% (1/104) for raw meat, egg, milk and vegetable, respectively. All of 79 isolates were serotyped into five serogroups: 1/2a or 3a (55.7%, n=44), 1/2c or 3c (21.5%, n=17), 1/2b or 3b or 7 (17.7%, n=14), 4a or 4c (3.8%, n=3) and 4b or 4d or 4c (1.3%, n=1). This research represents the different prevalence of L. monocytogens in raw-foods in Shanghai, and partial isolates belong to high-pathogenicity. Our results will provide the fundamental data for epidemiological monitoring of L. monocytogenes in Shanghai.

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  • Online: November 11,2020
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