Objective： To investigate the effect of fruits and vegetables on hypertension. Methods： 5 kinds of fruits and vegetables （broccoli， garlic， spinach， oyster mushroom and kiwifruit） were selected for inhibiting angiotensin converting enzyme （ACE） activity on the basis of pre-experiment. The experiments of water extraction， pepsin hydrolysate and ethanol extraction of samples， and mutual mixing and simulated body digestion inhibiting the activity of ACE was carried out. The content of hippuric acid was detected by high performance liquid chromatography （HPLC） to respond the effect of the extracts on the activity of ACE. Result： The water-soluble substances and pepsin hydrolysate of fruits and vegetables significantly inhibited the ACE activity， whereas some ethanol extracts could promote the ACE activity. Moreover， the water extracts of kiwifruit， Pleurotus ostreatus and pepsin hydrolysates of garlic and spinach strongly inhibited ACE activity. Both of water extract and pepsin hydrolysate of broccoli strongly inhibited the activity of ACE， but the inhibitory effect of its aqueous extract decreased greatly after dilution. The polypeptide of broccoli might have the strongest ability to inhibit ACE activity. Triple mixing compound of the pepsin hydrolysates of broccoli， garlic and spinach has synergistic effect. The water extracts of kiwifruit and Pleurotus ostreatus combined with spinach pepsin hydrolysates also had synergistic effect， whereas the water extracts of kiwifruit and Pleurotus ostreatus had antagonism with the pepsin hydrolysates of garlic and broccoli. The pepsin hydrolysates of broccoli， garlic and spinach remained high ACE inhibitory activity after simulated digestion. Broccoli polypeptide showed the strongest anti-digestibility. Conclusion： Most fruits and vegetables have both lowering and raising blood pressure substances. Its lowering blood pressure ability was stronger than raising. Polypeptide might be the main substances in fruits and vegetables that lower blood pressure. There are some water-soluble lowing hypertension substances， while some weak polarity substances might play a role in raising blood pressure. Thus， some good antihypertensive fruits and vegetables -based foods may help lower high blood pressure.