Perca fluviatilis were treated by different treatments （soaking in water or 3 mg/mL garlic powder solution， boiled with distilled water or 3 mg/mL garlic powder solution） and the content of adenine， guanine， hypoxanthine， xanthine and total purine were determined using high performance liquid chromatogram（HCLP） method. The results showed that boiling treatment could effectively decrease the content of total purines， by contrast， the effect of soaking treatment was not obvious. The removal rates of purine increased after adding 3 mg/mL garlic powder in the boiling treatment as well as the group of soaking treatment purine content was lower than that in blank group. The highest removal rate of total purine in Perca fluviatilis was obtained by boiling 3 mg/mL garlic powder solution， which was followed by boiling in water， soak in 3 mg/mL garlic powder solution and soak in water. The results showed that the purine content of the sample decreased， when the boiling time was 12 minutes， while the taste was guaranteed.