Objective： The minimum inhibitory concentrations （MICs） for 15 antibiotics of 20 Bifidobacterium isolates were determined and the transfer of antibiotic-resistance genes was assayed. Methods： 20 strains of Bifidobacterium strains were tested for the minimum inhibitory concentration of 15 antibiotics by broth dilution method， and the specific primers were used to detect whether the strain contained drug resistance genes by polymerase chain reaction. Finally， the drug resistance gene transferred was tested by filter hybridization. Results： The MIC values varied significantly among the Bifidobacterium isolates， including those of the same species. All of the Bifidobacterium isolates were susceptible to ampicillin， gentamicin， streptomycin， and linezolid and intrinsically resistant to kanamycin； in contrast， the susceptibility to chloramphenicol， ciprofloxacin， clindamycin， erythromycin， neomycin， tetracycline， trimethoprim， and vancomycin varied among the isolates. PCR analysis showed that several Bifidobacterium isolates harbored tet（W）. However， filter-mating experiments indicated no transfer of antibiotic-resistance genes. Conclusion： Bifidobacteria are not involved in further emergence of antibiotic resistant bacteria and that they are safe enough in this issue.