To determine the taxonomic classification of reference strain CMCC（B） 40001， which cited in GB 4789.28-2013 National food safety standard Food microbiological examination： Quality requirements of medium and reagents， polyphasic identification approaches including morphological features， 16s rRNA sequence， MALDI-TOF MS， VITEK 2 COMPACT automatic microbial analysis system and API were used. Results indicated that CMCC（B） 40001 was identified as Bordetella bronchiseptica and inconsistent with the scientific name- Alcaligenes faecalis in GB 4789.28-2013.