The exploitation and utilization of turtle oil has a significant effect on the rational use of the by-product. In this study， referring to the drawbacks of traditional heating-assisted desorption in headspace solid phase microextraction-gas chromatography-mass spectrometry （HAD-HS-SPME-GC/MS） analysis of turtle oil volatile components， including long time heating， insufficient desorption， etc.， utilizing the benefits of laser technology in promoting the fast release of volatile components， a novel， fast， efficient and convenient laser-assisted desorption method was built， which was conducive to efficient release in a shorter period of time during the analysis of volatile components of turtle oil. The results showed that the experimental parameters were optimized， including laser power （10 W）， irradiation time （5 min）， sample addition amount （3 g）. A total of 53 volatile substances in the turtle oil were detected， and the main characteristic flavor compounds were nonanal， hexanal， heptanal， 2-nonanone， 2-undecone， 1-penten-3-ol， hexanol and so on. Compared with the conventional HAD-HS-SPME-GC/MS， the LID-HS-SPME could significantly reduce the extraction time to 5 min from a previous demand of 30 min， with similar fingerprint profiles and more volatile molecular species. Afterwards， the method was validated in terms of sensitivity， precision， and recovery， which indicated the LID-HS-SPME GC/MS method was efficient and remarkable. The results of this study had the reference value for rapid detection of volatile components in liquid samples.