Some bamboo products are often used for food packaging or food contact materials. In order to study the antiseptic effect of chitosan on bamboo. The experiment of chitosan（food additive） inhibiting the growth of fungi， which caused bamboo products to go moldy was carried out. Fungi was separated and purified from moldy bamboo product. Different concentrations of chitosan treated bamboo product at ambient temperature， or 100 ℃， or with ultrasound assisted soak. After treatment they were inoculated with fungi， and the total number of colonies was determined. Viscosity changes and fungal inhibition of chitosan treated by different high temperatures were determined. Chitosan soaking treatment can increase the anti-corrosion of bamboo. Combining with heating soak， the antifungal effect will be greatly improved. Ability of bamboo materials absorbing chitosan will fine when it was soaked at less than 60 ℃. The optimum treatment was bamboo product soaked with 5 mg/mL chitosanat 60 ℃ for 24 h.