To investigate whether the acid-resistant candidate genes ATPase and FtsH with different expression trends under stress conditions can improve the acid resistance of strains， the real-time quantitative PCR （RT-qPCR） was used to determine the relative expression levels of mutagenized Oenococcus oeni， acid-resistant strain b1 and acid-sensitive strain b2， in ATB medium at pH 4.8 （optimal condition） and pH 3.0 （stress condition）. Then their expression trends were analyzed. ATPase and FtsH were cloned from b1， which were named ATPase1 and FtsH1； ATPase and FtsH were cloned from b2， which were named ATPase2 and FtsH2. Next analyzed the sequence differences. The function of ATPase1， ATPase2， FtsH1 and FtsH2 were verified in Lactobacillus plantarum （L. plantarum） XJ25， to establish recombinant L. plantarum a1， a2， f1 and f2. The growth ability of recombinant L. plantarum was determined in MRS medium at pH 3.2， using L. plantarum con containing empty vector as a control. Quantitative results showed that ATPase was significantly up-regulated in both strains b1 and b2； FtsH was unchanged in b1 and significantly up-regulated in b2. ATPase1 and ATPase2 have three non-synonymous mutations in the functional domain， and FtsH1 and FtsH2 have one non-synonymous mutation in the transmembrane domain. The functional verification results showed that the growth ability of a1 and a2 was stronger than con， and there was no difference in the growth ability between a1 and a2； the growth ability of f1 was stronger than that of con， and the growth ability of f2 was not different from that of con. It can be seen that ATPase1， ATPase2 and FtsH1 can all improve the acid resistance of the strain.