The ultra performance liquid chromatography-tandem mass spectrometry method was established to quantify the bovine serum albumin in bovine dairy products accurately based on targeted proteomics. Qualitative analysis of theoretical peptides was carried out using high-resolution mass spectrometry （Q-Orbitrap） and protein software. Based on the the specificity and stability of peptides and the dynamics curve of enzymatic hydrolysis， LVNELTEFAK and HLVDEPQNLIK were selected as the specific proteotypic peptides for quantitative determination of bovine serum albumin. The detection limits of both peptides were less than 1 mg/100 g. And the recovery rates were between 87.9% and 101.1%， the intra-day and the inter-day precisions were less than 7.6%. The detection of 12 infant formula and whey powder showed that the method was suitable for the quantitative detection of bovine serum albumin in dairy products. It provides a technical foundation for quality monitoring of infant formula.