Objective： To isolate， identify and analysis the strains with high cholesterol-lowering activities from traditional ferments. Methods： Strains were screened by MRS， M17 and PDA culture media， and explore the tolerance of bile salt， acid resistance， hydrophobicity， self-cohesion ability， bile salt hydrolytic enzyme activity and cholesterol lowering properties. Results： 51 strains were isolated from ferment， among which S-8， M-7 and M-16 had better cholesterol-lowering capacity and the cholesterol reduction rates were 25.43%， 37.06% and 47.41%， respectively. The tolerance of bile salt tolerance were 42.09%， 65.64%， 22.80%； S-8 strain had the highest hydrophobicity in xylene， and the hydrophobicity was up to 35.91%， and the hydrophobicity of M-7 in chloroform was 45.22%. Both strains have good acid resistance and self-cohesion， after standing for 24 hours， the self-cohesion ability was more than 80%. The cholesterol-lowering effect was investigated. It was found that the intracellular enzymes were mainly responsible for this activity and had 7 times higher specific activity than the extracellular ones. Conclusion： Through the morphological observation and 16S rDNA molecular analysis， the S-8 strain was identified as L. paracasei strain， the other two strain were identified as Staphylococcus hominis strain. It is possible to further study microorganism on the regulation of lipid metabolism.