Objective： To investigate the protective effect of hydrolyzed egg white short chain polypeptide（HEpep） on LPS-induced colitis in rats. Method： 60 male SD rats were randomly divided into 4 groups： control group， model group， and high or low-dose of HEpep group. Rats in HEpep groups were administrated orally for 7 days at 2 g/（kg·d） or 1 g/（kg·d）， while the control and model group were treated with normal saline synchronously. On day 7， all rats were intraperitoneally injected with LPS of 10 mg/kg. 8 h after administration， blood and colon tissues were collected. Apoptosis and pathological changes were observed by HE staining and TUNEL staining. The levels of inflammatory factors-α and interleukin-6 in serum and tissue were detected by ELISA. Superoxide dismutase （SOD）， malondialdehyde （MDA） and catalase（CAT） in serum and tissues were tested. Results： HEpep significantly alleviated LPS-induced inflammatory response in colon tissues， relieved the pathological injury and apoptosis， reduced the levels of TNF-α and IL-6 and oxidative stress injury in tissues and serum. Conclusion： HEpep could alleviate the inflammatory response in colon tissues caused by LPS， and the mechanism is related to anti-inflammation， anti-oxidation and anti-apoptosis.