Objective： This paper aimed to evaluate the probability of liver cancer combination and intensity of combined liver injury induced by AFB1+MC-LR at low dose and sub-chronic conditions， and to promote the understanding the threshold and occurrence possibility of liver toxicity co-exposure to AFB1+MC-LR at dietary scenarios， the results were helpful for risk control（e.g. MRLs setting）. Methods： Based on the male wild-type C57BL/6 mice， CI model combined with scenarios extrapolating was used to predict the combined risk of hepatoma induced by AFB1+MC-LR at low dose and sub-chronic condition. And then， according to the expression of 5 typical targets in serum and liver， the combined effects of liver injury were explored. Results： Within dose range of 0-20 mg/kg bw， the CI index was in the range of 0-1.5， which showed the probability of additive/synergistic effect was relatively higher. With the equal effect ratio， the overall expression of combined group related with five indicators was slightly higher than that of single component in the serum and liver （P<0.05）， which was also confirmed by principal component analysis， the probability of synergistic effect was relatively higher than antagonistic and simple additive effects. With unequal effect ratio， except TBIL and AFP in serum， the change of any single component will affect the expression of combined toxicity （P<0.05）， while the change of single dose in liver will significantly affect the expression of combined toxicity （P<0.001）. Conclusion： The results showed that early liver injury induced by AFB1+MC-LR was slight synergistic effect at low dose and sub-chronic condition， and the change of any single mycotoxin can affect the expression of combined liver injury in mixtures. It should be paid attention to in the standard making and supervision.