Polysaccharides from Plantago asiatica L have good activities in intestinal function regulation， but there are few reports on their different effects because of the diverse structure. In this paper， high viscous arabinoxylan （PLCP）， low viscous arabinoxylan （PLCP-M and PLWE-H）， and non-arabinoxylan （PLWE-L） were prepared from the seeds of Plantago asiatica L. Animal experiments were applied to elucidate their effects on the intestinal function regulation of mice. The results showed that the intake of PLWE-L significantly increased the concentration of SCFA in colon and cecum contents， and increased the water content of feces. High viscous PLCP was more likely to play a role in the colon， which included increasing the concentration of total SCFAs in the colon， lowering pH value and improving water retention. While the low viscous components （PLCP-M and PLWE-L） probably played certain effects in the cecum. Therefore， there are some differences in effects of the four fractions on intestinal function regulation in mice. This study would provide theoretical support for the in-depth study on intestinal health of Plantago asiatica L. polysaccharide， and provide theoretical basis for the further development of related products.