In order to clarify the hypolipidemic effect of Auricularia auricula and Auricularia auricula polysaccharides and its correlation with intestinal flora， 5-week-old healthy male SD rats were used as experimental subjects， which were divided into normal control diet group， high-fat diet group， Auricularia auricula intervention high-fat diet group and Auricularia auricular polysaccharide intervention high-fat diet group for 6 weeks. During the period， rats' body weight， food intake， and serum biochemical indicators were measured， liver， cecum contents， and feces were collected for pathological analysis and intestinal flora detection after dissection. The results showed that the intervention of Auricularia auricula and Auricularia auricula polysaccharides can significantly reduce the serum total cholesterol and low density lipoprotein content， increase the high density lipoprotein content， promote liver lipid metabolism， reduce the accumulation of fat in the liver of high fat diet rats， and the intervention of Auricularia auricular is the most significant； Both Auricularia auricula and its polysaccharides intervention significantly increased the content of short chain fatty acids in the cecum， it is speculated that it may be possible to increase the abundance of short chain fatty acid producing flora such as Parabacteroides and Bacteroides to produce beneficial shorty chain fatty acids to promote blood lipid metabolism； The two also significantly increased the abundance of Puniceicoccaceae or Asaccharobacter in the feces of rats， significantly reduced the abundance of pro-inflammatory bacteria Prevotella， and alleviated hepatitis and other inflammations and diseases caused by high-fat diet； Auricularia auricular also regulates the abundance of Ruminococcaceae related to obesity， which may be the reason for its more significant preventive effect on hyperlipidemia. This study clarified the mechanism of hypolipidemic effect of Auricularia auricula and Auricularia auricula polysaccharides from the aspects of intestinal flora regulation， and provided suggestions for dietary intervention to regulate the intestinal flora to improve blood lipid metabolism and feeding patterns.