The cDNA fragments of Calpain-1， Calpain-2， Calpastatins， MuRF-1 and MuRF-2 in Pseudosciaena crocea were amplified and quantified by RACE-PCR and RT-PCR. Some partial coding sequence for large yellow croaker's five genes was determined and deposited in GenBank， we obtained the accessions and partial coding sequences as follow： Calpain-1（506 bp， no. KF527412）， Calpain-2 （425 bp， no. KF527413）， Calpastatin （724 bp， no. KF527409）， MuRF-1 （401 bp， no. KF527410） and MuRF-2 （474 bp， no. KF527411）， respectively. The analytical results of BLAST showed that the partial nucleotide sequence of Calpain-1 cDNA（506 bp） has 89% similarity with Oreochromis niloticus Calpain-1 （XM_003441017）. The partial cDNA sequence for Calpain-2 is 425 bps long， and the sequence is also closely related to the known Calpain-2 from other species as it shares 89% similarity with Hippoglossus hippoglossus Calpain-2 （GQ327966）. The nucleotide sequence of Calpastatin consists of 724 bps， and it displayed high sequence homology with the previously reported Calpastatin in other species with 94% similarity with Dicentrarchus labrax（FQ310506）. The nucleotide sequence of MuRF-1 cDNA is 401 bps in length and the sequence shows 82% similarity with Salmo salar （NM_001279046）. The cDNA sequence for MuRF-2 is 474 bps， and it shares 84% similarity with Oreochromis niloticus（XM_003458136）. In our study， the four gene-specific mRNA levels had either a longer post-mortem interval or a slower decreasing speed in the TP samples than in those in the control samples， except that no differences were observed in the mRNA level of the Calpain-2 gene when assessed with respect to the post-mortem storage time. The preservation effect of tea polyphenols（TP） on large yellow croaker fillets is partly due to the effect of TP on the corresponding mRNA stability fillets， thus effectively controlling the freshness of fish during refrigeration.