In this paper， the light scattering technique and Stokes-Einstein equation were used to study the interaction between lys， BLG， BSA， OVA and polystyrene microspheres（A）， polystyrene microspheres-carboxyl group （B）， polystyrene microspheres（C）， and to screen out the suitable colloidal particles. Moreover， an effective microcosmic method for measuring solution viscosity is optimized， and a fast and convenient method for monitoring microrheological behavior of food system is added.