Objective： Lactobacillus paracasei is a kind of lactic acid bacteria with potential probiotic function， it has a vast theoretical basis and extensive application scenarios. In this study， Lactobacillus paracasei PC-01 isolated from sour yak milk of Longren Township， Dangxiong County， Lhasa， Tibet， was used as the research object to evaluate its potential probiotic characteristics and safety. Methods： The survival rate of Lactobacillus paracasei PC-01 was 88.60% after cultured in simulated artificial gastrointestinal fluid for 11 h. The delay time of bile salt tolerance was 0.34 h. The safety evaluation results showed that the strain was sensitive to six antibiotics， and resistant to vancomycin and clindamycin. Hemolysis test was negative and animal experiments further confirmed that Lactobacillus paracasei PC-01 had no side effects. Conclusion： Lactobacillus paracasei PC-01 has potential probiotic properties and safety， which provides a reference for its wide application.