Screening, Identification and Probiotic Characteristics of Bacteriocin-producing Strains of Lactic Acid Bacteria from Goat Milk
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    Abstract:

    In this study, three lactic acid bacteria (LAB) with antimicrobial activity against common food-borne pathogens (Staphylococcus aureus, Listeria monocytogenes and Bacillus cereus) were obtained from goat milk, and their antimicrobial properties and potential probiotic characteristics were described. Those strains were identified as Enterococcus faecalis (termed as DH9003 and DH9012) and Lactococcus lactis (termed as DH9011), and their antimicrobial products showed typical bacteriocin traits, such as being of heat-stable and proteinase nature. Bacteriostatic activity of concentrated bacteriocin produced by those LAB was found at low mass concentration (MIC50 and 4MIC50), while complete inhibition activity against Lis. monocytogenes was found from two En. faecalis strains (DH9003 and DH9012) at high mass concentration (16MIC50). Besides of that, all those LAB were of no-hemolytic activity, sensitivity to ampicillin (50 mg/mL) and streptomycin sulfate (100 mg/mL), and resistance to bile, artificial simulated intestinal tract and gastric juice at different pH level (2.5, 3.0, 3.5), β-galactosidase activity. In addition, all those LAB present auto-aggregating phenotype with ranged from 30%-55%. DH9003 and DH9012 showed good co-aggregation ability with Lis. monocytogenes and E.coli (52.6%, 63.2% and 68.5%, 57.6%, respectively), while less co-aggregation ability between DH9011 and Lis. monocytogenes was obtained as 15.6%, and no co-aggregation with E.coli. The results in our study demonstrated that all those LAB were of strong antimicrobial activity, tolerance to bile and simulated gastrointestinal environments, adhesion ability, safe characteristics. Therefore, those LAB are considered as potential probiotic candidates with huge application values.

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  • Online: December 17,2021
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