Phytosterols have been widely concerned for their efficacy in lowering cholesterol,anti-tumor,anti-inflammatory and anti-oxidation.In order to find out the composition of phytosterols in cauliflower,both free and three kinds of conjugated phytosterols were separated by solid phase extraction,and then the species and structure of phytosterols in cauliflower were identified and analyzed by UPLC-Triple-TOF/MS.The results showed that the free phytosterol(FS) in cauliflower were stigmasterol,campesterol,lupeol and β-sitosterol. Campesterol linoleate,stigmasterol oleate and β-sitosterol oleate were identified as three phytosterol esters(SE).Campesterol glucoside and β-sitosterol glucoside were identified as phytosterol glycosides (SG).Acylated sterol glycosides (ASG) include campesterol glucoside palm oleyl ester,campesterol glucoside eicosapentaenoic acid ester,stigmasterol glucoside linolenic acid ester,β-sitosterol glucoside eicosapentaenoic acid ester and β-sitosterol glucoside linolenic acid ester.