On the basis of establishing the simulated reaction system of non-enzyme gluco-sylation protein and reducing sugar in vitro， the inhibitory effect of turtle antioxidant peptide on non-enzyme gluco-sylation(NEG) reaction and advanced glycation end products (AGEs) was investigated. The results showed that the mass concentration of anti-oxidation peptide was 1.00 mg/mL， and the inhibition rate of NEG was 68.79% when incubated for 21 days， the inhibition rate of AGEs was 89.35% when incubated for 28 days. Infrared spectroscopy results proved that antioxidant peptide of turtle could effectively inhibit the production of AGEs in non-enzymatic gluco-sylation reaction.