The objective of this study was to obtain the optimal technology of ultrasonic-assisted extraction of collagen from camel skin， and the extracted collagen was characterized and analyzed. In the experiment， the extraction rate of collagen was used as an indicator to explore the effects of enzyme loading， solid-liquid ratio， ultrasonic power， ultrasonic treatment time and total extraction time on camel skin collagen. After determining the ultrasonic power， on the basis of one-factor-at-a-time method， combined with response surface analysis， the best extraction conditions: enzyme loading of 4.5%， solid-liquid ratio of 1∶23， ultrasonic treatment time of 20 min， total extraction time of 51 h， and the actual extraction rate of collagen was (41.99±1.57) %. The SDS-PAGE analysis suggested that the extracted collagen contained three characteristic chains of type I collagen. Ultraviolet spectroscopy analysis was further inferred to be a typical type I collagen. The Fourier transform infrared spectroscopy analysis confirmed that collagen had a complete triple helix structure. Scanning electron microscopy observation revealed that the extracted collagen was in the form of fibrous with intertwined structure. Meanwhile， the Zeta potential of pH 2~9 of collagen was measured， and the potential value showed a downward trend in this range. The isoelectric point was 6.7.