The aim of our study was to investigate the protective role of camel milk(CM) on dextran sulfate sodium (DSS)-induced colitis in mice， which might provide a new entry point for the research and treatment of colitis. In this study， ulcerative colitis was induced by oral administration of 2.5% DSS for 7 days in drinking water. A total of 60 male C57BL/6 mice were randomly divided into 4 groups: control group， model group， CM group (10 mL/kg) and CM+DSS group. The protective effect of CM was assessed by weight change， disease activity index(DAI)， histopathological scores and immunochemistry. Detect the level of inflammatory cytokines and myeloperoxidase (MPO) activity in colon and serum by ELISA and real-time fluorescent quantitative PCR to further reflect the influence of camel milk on inflammation. Compared with the model group， CM supplement decreased weight loss and DAI (P>0.05)， also significantly reduced the levels of MPO activity and inflammatory cytokine(IL-1β， IL-6 and IL-10) levels in serum and colon tissue (P<0.05). Meanwhile， CM can relieve the colon shorten symptoms(P<0.05) and protect colon tissue structure. Camel milk has the effects of inhibiting neutrophil infiltration， reducing inflammation and protecting the structure of colon tissue， and has a certain protective effect on DSS-induced colitis damage in mice.