Objective: To investigate the protective effects of different doses of WEG on renal injury in rats with acute nitrite poisoning. Methods: Thirty six rats were divided into blank group， model group， wheat germ globulin group (80 mg/kg)， low-， medium-， and high-dose groups (40， 80， 160 mg/kg) and vitamin C (80 mg/kg) groups. The rats were continuously intervened for one week. After 12 hours of the last administration， the rats were challenged with 60 mg/kg NaNO₂. The changes of antioxidant activity， apoptotic factor content and partial renal function were observed and compared， and the morphological changes of renal tissue were observed by hematoxylin eosin staining under microscope. Results: Compared with the model group， different doses of wheat germ globulin can significantly reduce the kidney coefficient， increase the contents of superoxide dismutase (SOD)， glutathione (GSH)， catalase (H2O₂)， antioxidant capacity (T-AOC)， reduce the production of malondialdehyde (MDA)， reduce the levels of urea nitrogen (BUN) and creatinine (CR) (P<0.05)， and increase the contents of caspase 3 and caspase 8 in kidney in addition， the degree of renal degeneration and necrosis was significantly reduced. Conclusion: Wheat germ globulin has potential protective effect on renal toxicity caused by nitrite. It can effectively improve the antioxidant activity of renal tissue， enhance the anti-inflammatory stress capacity， promote the balance of anti-inflammatory factors and pro-inflammatory factors， and has protective effect on renal injury caused by acute nitrite poisoning.