Biofilm is a bacterial polymer formed by bacteria contacting with surfaces of objects to resist adverse environment. Spoilage bacteria can form mature biofilm and attach to the surface of aquatic products， which shows stronger corruption and is difficult to remove. Therefore， it is necessary to comprehensively understand biofilms of spoilage bacteria. In this paper， the composition and formation process of spoilage bacteria biofilm in aquatic products and the molecular regulation mechanism of biofilm of three strains (Shewanella， Pseudomonas fluorescens and Aeromonas hydrophila) were reviewed. In these bacteria， the formation of biofilms is generally regulated by cyclic diguanylic acid (c-di-GMP) and quorum sensing (QS). These regulatory systems inhibit or promote the formation of bacterial biofilm by changing the adhesion， motility and extracellular polysaccharides of bacteria. Finally， the novel methods of controlling the biofilm of spoilage bacteria were introduced， and some new methods applied to other strains were summarized in order to be used in spoilage bacteria. It can provide a theory reference for the regulation mechanism of biofilm formation and prevention measures of spoilage bacteria in aquatic products.