Objective: To investigate the synergistic antioxidant effects of burdock polysaccharide and chlorogenic acid on zebrafish. Methods: The combination of burdock polysaccharide-chlorogenic acid complex with the strongest synergistic antioxidation was selected by constructing a zebrafish oxidative damage model. At the same time， burdock polysaccharide-chlorogenic acid complex was fabricated based on the optimal ratio， and its structure was characterized and antioxidant activity in vivo and in vitro was evaluated. Results: When the mass ratio of burdock polysaccharide to chlorogenic acid was 1∶3， the complex was observed showing the strongest enhanced antioxidant effect in vivo. The average particle size of the complex was about 50-60 nm， and the zeta potential was (-6.36±0.80) mV. Infrared spectrum and scanning electron microscope results showed that an amorphous flake structure was formed between polysaccharide and chlorogenic acid， and hydrogen bonding may be the main driven force for complex formulation. In addition， the complex could significantly increase the activities of T-SOD， CAT and reduce the content of MDA in zebrafish， and effectively scavenge DPPH， ABTS+， OH-， O₂- free radicals. Conclusions: Burdock polysaccharide-chlorogenic acid complex can play a synergistic role in antioxidant， which provides a basis for the improvement of natural antioxidant.