In order to study the effect of micro-environmental modified atmosphere on bluberries during storage， micro-environmental modified atmosphere package (mMAP)， 1-methylcyclopropene (1-MCP) and micro-environmental modified atmosphere package combined with 1-methylcyclopropene (mMAP+1-MCP) were used to treat bluberries， and the untreated blueberries were used as control. The blueberries after treated were stored in cold storage (-0.5 ± 0.3) ℃， and fruit firmness， contents of cell wall polysaccharide， activities of cell wall degrading enzyme and key degrading enzyme gene expression were measured at 0， 20， 40 and 60 day during storage. The results showed that the three treatments could significantly reduce the soft fruit rate compared with the control group (P < 0.05)， and the effect of mMAP + 1-MCP treatment was the best. The fruit firmness of mMAP + 1-MCP treatment was significantly higher than other treatments(P < 0.05)， with higher content of cellulose and protopectin. The hemicellulose of it was higher than other treatments in the early stage of storage while the soluble pectin content remained lower in the early and medium stage. According to the analysis of cell wall degrading enzymes， the activities of cellulase(Cx)， polygalacturonases (PG) and pectinmethylesterases (PME) of mMAP+1-MCP treatment group were the lowest at 40 day of storage， and the activities of β-galactosidase (β-Gal) and α-L-arabinofuranosidase (α-Af) of mMAP+1-MCP treatment were significantly (P < 0.05) lower than those of other treatments during 60 day. Orthogonal partial least squares discriminant analysis (OPLS-DA) showed that the differences between mMAP+1-MCP treatment and other treatments were Cx and β-Gal， the expression of the two key cell wall degrading enzymes was analyzed and the results showed that mMAP+1-MCP treatment could effectively delay the time which peak value occurs of Cx and β-Gal genes during storage of blueberries. Therefore， the mMAP+1-MCP treatment could delay the time which peak value occurs of the gene expression of Cx and β-Gal and inhibit the activities of Cx and β-Gal in the late stage of storage， maintaining the contents of cellulose and pectin， and then slow down the softening of fruits.