Abstract:A method for rapid screening of pesticide residues in herbal tea by gas chromatography coupled with quadrupole time-of-flight mass spectrometry (GC-Q-TOF/MS) was developed. The target compounds in samples were extracted by ethyl acetate, cleaned by GCB and NH2 sorbent and determined by GC-Q-TOF/MS. The detection limits of 20 controlled pesticides were within 2.00-50.0 μg/kg and the limits of quantitation were 5.0-150 μg/kg. The average recovery of the sample ranged from 73.47%-106.3% at three different spiked levels(25, 50, 125 μg/kg), with RSDs (n=6) of 0.91%-9.11%. Moreover, the acquired data was compared with the library using the accurate mass of characteristic ion, ratio of product ion and isotopic distribution of characteristic ion to screen pesticide residues in herbal tea. Screening and confirmation was achieved by library search, and the minimum concentration with a score(≥80) was used as the screening limit of the method without reference standards. This method is high efficiency, sensitive and accuracy, and can meet the requirement for non-target rapid screening of pesticide residues in herbal tea.