Objective: To explore the regulatory effect of Lycium barbarum-Glycyrrhiza liqueur on immune function and antioxidant status in mice. Methods: Immunosuppressed mouse model was constructed， the mice were randomly divided into 6 groups: control check(CK)， cyclophosphamide model group (CY)， base wine control group and high， medium and low-dose Lycium barbarum-Glycyrrhiza liqueur groups， normal saline or 20， 10， 5 mL/kg bw Lycium barbarum-Glycyrrhiza liqueur were given orally for 14 days. After the last injection， the animal mass， thymus index， spleen index，phagocytic index， immunoglobulin G(IgG) and immunoglobulin M(IgM) content， interleukin-6 (IL-6)， interferon-γ (IFN-γ) and tumor necrosis factor-α(IFN-α) were determined in each group. The activity of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px)， and the concentration of malondialdehyde (MDA) in serum were determined in each group. Results: compared with the model group， the contents of IgG， IgM， IL-6， IFN-γ， IFN-α， GSH Px， SOD activity and MDA concentration were significantly changed in the medium dose group. The results showed that Lycium barbarum and licorice wine could effectively improve the immune regulation and antioxidation of immunosuppressive mice induced by cyclophosphamide.