This experiment was conducted to investigate the effects of low-temperature alive transportation on the blood biochemical and muscle physicochemical indexes of sea bass. Firstly， the effects of temperature， salinity， density and holding time on the survival rate of sea bass after transportation were measured. Secondly， the sea bass were cooled down to the aliven transportation temperature 12， 16 ℃ and 20 ℃ at a cooling rate of 3 ℃/h. Sea bass that were not transported at room temperature were used as the control group， the water quality indexes， blood biochemical and muscle physicochemical indexes were measured at the 12， 24， 36， 48， 60 and 72 hour of transportation and 12 hours after the end of transportation. The results showed that the survival rate was highest in the conditions of 12 ℃， 16‰ salinity， 1 ∶ 10-1 ∶ 8 fish-water ratio and 36 h temporary storage time. During the whole keeping alive transportation process， the water quality showed a significant decreasing trend with the increase of temperature(P<0.05). Serum lactate dehydrogenase， aspartate aminotransferase and alanine aminotransferase activities and cortisol levels were significantly increased (P < 0.05)， and glucose and heat shock proteins showed a trend of first increase and then decrease. After 12 h of recovery at the end of transportation， the heat shock proteins， lactate dehydrogenase， aspartate aminotransferase and alanine aminotransferase activities of sea bass in the 12 ℃ group returned to those of the control group. The total protein and lactate levels in the muscles showed a significant increasing trend， and glycogen， hardness and chewiness decreased significantly (P < 0.05). There were no significant changes in pH value， water-holding capacity and springiness of sea bass muscles before and after the alive transportation (P > 0.05). The optimal holding temperature for long-distance transportation of sea bass was 12 ℃. The results of this study provided a reference for the alive transportation of sea bass and other marine fishes.