Objective: To investigate the anti-ageing effects of the complex of collagen peptides combined with Tremella fuciformis polysaccharides (CPTP) on skin aging in D-galactose induced aging animal model and its mechanism. Method: D-galactose-induced aging mouse model were divided into three groups: Model group (administration of physiological saline)， CP group [administration of collagen peptide in a dose of 830 mg/(kg·d)]， and CPTP group [administration of a complex of collagen peptide and T. fuciformis polysaccharides in a dose of 830 mg/(kg·d) and 100 mg/(kg·d)， respectively]. Adult mice were set as normal group. Intraperitoneal injection (i.p.) daily with physiological saline were conducted for mice in Normal group， while i.p. daily with D-galactose in a dosage of 120 mg/(kg·d) were performed for those in remaining groups. All mice were given intragastric administration for eight weeks and then killed. The concentrations of hydroxyproline (HYP) and hyaluronic acid (HA) in skin， the activities of anti-oxidant enzymes and the production of MDA in tissues， the contents of inflammatory cytokines in serum， the productions of intestinal SCFAs as well as the pH value were measured. Results: CPTP remarkably improved the contents of HYP and HA in skin (P < 0.01) and its efficacy were better than that of collagen peptide alone (P < 0.01)， obviously increased the activities of GSH-Px and SOD in serum， liver and heart， while reduced the concentrations of MDA (P < 0.01)， and inhibited the secretions of IL-1β and TNF-α (P < 0.01)， but had no significant effects on the concentrations of SCFAs in the colon though alterations in pH in colon were observed， compared with model group. Conclusion: The improvements of the loss of HYP and HA in aging skin in D-galactose induced aging mice in response to CPTP treatment might be due in part to an increase in the activities of antioxidant enzymes and a reduction in secretion of inflammatory cytokines. These findings may help to facilitate the application of CPTP in functional foods for beauty.