Identification of Flavonoids from the Flavedo of 23 Citrus Cultivars Fruit and Their Antioxidant Activity
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(Laboratory of Fruit Quality Biology, Zhejiang University/ Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Ministry of Agriculture and Rural Affairs/ Horticultural Products Cold Chain Logistics Technology and Equipment National-Local Joint Engineering Laboratory/ Zhejiang Provincial Key Laboratory of Integrative Biology of Horticultural Plants, Hangzhou 310058)

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    Abstract:

    In this study, the flavedo of 23 Citrus varieties were selected as materials, and the components and antioxidant capacity of flavonoids were investigated by Liquid Chromatography-Mass spectrometry(LC-MS). The results showed that, the total phenolic content of the flavedo varied from 4.38 mg GAE/g DW to 21.10 mg GAE/g DW; and the varieties with the strongest and weakest comprehensive antioxidant capacity in the flavedo were 'local early' and 'Aiyuan 38'. Using the LC-MS to identify the flavonoids in the flavedo of 23 Citrus varieties, we identified a total of 53 flavonoids, including 5 flavonoid-C-glycoside, 4 flavonoid-O-glycoside, 12 flavanone-O-glycoside, 32 polymethoxyflavone (PMFs). Compared with the existing literature, two flavonoids, phloretin-3',5'-di-C-glucosid and hesperitin-7-O-rhamonoside, were reported for the first time in Citrus. The main flavanones contained in the flavedo are eriocitrin, neoeriocitrin, narirutin, hesperidin, and didymin. The main PMFs in the flavedo are nobiletin, tangeretin, 5HPMF and sinensetin. In conclusion, this article further supplemented information on the components and antioxidant activities of different Citrus varieties, which provided scientific guidance for Citrus consumption and set a theoretical basis for bioactivity studies.

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History
  • Received:December 13,2021
  • Revised:
  • Adopted:
  • Online: January 09,2023
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