Analysis on the Evolutionary Changes of Fungus Fermentation with the Characteristics of Fu Brick Tea Based on Microbiome Amplicator ITS
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(Department of Tea, College of Horticulture, Henan Agricultural University, Zhengzhou 450046)

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    Abstract:

    Fu brick tea is a kind of dark tea fermented with microorganisms. The fungal potpourri is the brick tea unique aroma quality, formed with fermentation is directly related to fungi flora succession. To reveal the characteristics of fungi flora of Fu brick tea fermentation succession law, the dark tea and white tea were made from Yabukita tea Camellia sinensis (L.) O. Ktze from Shenlin Tea Industry Development Co., Ltd., Luoshan County, Xinyang City, Henan Province. After that, the Fu brick tea and white tea brick are respectively made from flowers in the same place. Microbiome amplifiers ITS (Internally Transcribed Spacer) were used to analyze the succession law of fungus with fermentation characteristics of Fu brick tea. The results showed that: 1) There was no significant difference in the fungal diversity of different tea species during the fermentation process, but the difference was caused by the different variation rules of characteristic fungal abundance. 2) The piles-fermentation changes the flora structure and microbial growth environment of Fu brick tea, which directly leads to the significant difference in the flora succession of fermentation fungi between Fu brick tea and other types of tea. 3) Succession law of fungus flora with fermentation characteristics of Fu brick tea: In the early stage of Fu brick tea blossoming, Aspergillus_niger, Aspergillus_restrictus, Aspergillus_sydowii, Penicillium_steckii, Penicillium_brocae, Debaryomyces_hansenii with higher abundance affected by piles-fermentation, showed abundance peak on the 6th day of fermentation. After that, on the 9th day of fermentation, the abundance peaks of Eurotium, Meyerozyma_caribbica, Wickerhamomyces_anomalus, and other fungi appeared. Aspergillus_niger, Debaryomyces_hansenii, Aspergillus_restrictus maintains abundance. On the 12th day of fermentation, Aspergillus_pseudoglaucus had its peak abundance, Aspergillus_niger continued to maintain its abundance. By the end of fermentation day 22, Eurotium was finally the dominant species.

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History
  • Received:January 10,2022
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  • Online: February 24,2023
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