Analysis of Metabolite Differences during the Browning Process of Yellow Peach Based on Untargeted Metabolomics
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(1.School of Agriculture and Biotechnology, Hunan University of Humanities, Science and Technology,Loudi 417000, Hunan;2.Institute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences, Key Laboratory of South Subtropical Fruit Biology and Genetic Resource Utilization, Ministry of Agriculture and Rural Affairs, Guangdong Provincial Key Laboratory of Tropical and Subtropical Fruit Tree Research, Guangzhou 510640)

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    Abstract:

    In this study, untargeted metabolomics method was used to identify and analysis the differences of metabolites during the browning of yellow peach. Yellow peach fruit were taken as experimental materialsl, and their metabolites were analyzed by principal component analysis (PCA), cluster analysis and orthogonal partial least squares discriminant analysis (OPLS-DA). The results indicated that pulp browning was significantly inhibited by PE packaging treatment. A total of 991 metabolites were detected in yellow peach fruit. 206 significantly up-regulated metabolites and 26 significantly down-regulated compounds were identified in the CK 12 d vs CK 0 d samples. While 145 significantly up-regulated metabolites and 58 significantly down-regulated compounds were identified in the PE 12 d vs CK 0 d samples. There were 13 HMDB metabolic pathways by using HMDB database. The first top 6 pathways were phenylpropanoids and polyketides, lipids and lipid-like molecules, organoheterocyclic, organic acids and derivatives, benzenoids and organic oxygen compounds. KEGG results showed that differential metabolites mainly distributed in 10 KEGG metabolic pathways, the first top 4 pathways were biosynthesis of other secondary metabolites, amino acid metabolism, metabolism of cofactors and vitamins and metabolism of other amino acids. This study revealed differential metabolites during the browning process of yellow peach, which could provide a theoretical reference for improving the quality of yellow peach fruit.

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  • Received:February 02,2022
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  • Online: March 22,2023
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