In this study， 16 strains of Lactobacillus were analyzed for their antibacterial ability against pathogenic bacteria Staphylococcus aureus， Salmonella typhimurium and Escherichia coli， adhesion and intervention of pathogenic bacteria to Caco-2 cells， resistance to acid and bile salt stress and regulatory activity to immune cells. The results showed that the diameter of inhibition zone corresponding to the cell-free supernatant of L. plantarum KLDS 1.0328 was more than 15 mm. Using Lactobacillus rhamnosus LGG as control， six Lactobacillus strains with stronger or equivalent adhesion ability to Caco-2 cells in vitro were screened. Four strains with strong inhibitory ability to Caco-2 cells were further screened based on the competition， exclusion and displacement tests. The inhibition rates of L. plantarum KLDS 1.0328 for competition of S. typhimurium was (72.52 ± 4.94)%. Additionally， it is confirmed that L. plantarum KLDS 1.0328 has strong tolerance to acids and bile salts， has a good regulatory effect on immune cell activity， and has the potential for further development and application in food industry.