Zein/ pectin composite colloidal particles were prepared by anti-solvent combined with heat treatment， which were further used to construct cinnamon essential oil-encapsulated Pickering emulsion by high-speed homogeneous emulsification. The effect of zein/pectin mass ratio， pH value and the proportion of oil phase on the ζ-potential， average particle size， particle size distribution (PSD) and encapsulation efficiency of the emulsion were investigated， and the response surface methodology (RSM) was used to optimize the preparation process. The RSM results showed that the proportion of oil phase was a critical factor affecting Pickering emulsion and the interaction between pH value and the proportion of oil phase was the most significant. When zein/pectin mass ratio was 10 ∶ 4，pH value was 3 and proportion of oil phase was 50%， the comprehensive score of the emulsion could reach 85.52. Under the above optimized conditions， the encapsulation efficiency， average particle size， PSD and ζ-potential of Pickering emulsion were(82.14 ± 4.86)%， (15.69 ± 0.17) μm， (1.277 ± 0.011) μm， and (-30.75 ± 1.63) mV， respectively. The experimental results provide an experimental reference for the preparation of Pickering emulsion containing plant essential oils.