Different lighting time affects the content of bioactive substances in postharvest kale sprouts. The contents of glucosinolates， vitamin C， total polyphenols， chlorophyll， carotenoids， and total antioxidant capacity in Chinese kale sprouts were analyzed during postharvest storage at room temperature after different treatments including shading treatment group (0 h light / 24 h darkness， 8 h light / 16 h darkness and 12 h light / 12 h darkness) and supplementary light treatment group (24 h light / 0 h darkness)， and the natural photoperiod (16 h light / 8 h darkness) was used as control. The results showed that total glucosinolates content in shading treatment T1 (0 h light / 24 h dark) increased significantly by 27.96% and 19.73% respectively when stored for 2 d and 3 d compared with the control， and the shading treatment T2 (8 h light / 16 h dark) increased remarkably by 27.70% and 10.88%， respectively. In addition， shading treatment effectively maintained the contents of chlorophyll and carotenoids in Chinese kale sprouts during storage. The postharvest light time had no significant effect on the total polyphenol content and antioxidant capacity. The research results provide a theoretical basis for setting the light time reasonably to postpone the decrease of Chinese kale sprouts after harvest， which is potential in maintaining the quality of Chinese kale sprouts during the postharvest period.