Guizhou black tea has excellent quality due to its ecological advantages. In order to have an insight into the quality characteristics of Guizhou black tea， non-targeted metabonomics analysis for the nonvolatile tea metabolite of three representative of Guizhou black tea(Zunyi red tea、 Pu'an red tea、 Shiqian moss tea)was conducted by ultra-performance liquid chromatography-quadrupole-orbitrap high resolution mass spectrometry (UPLC-Q-Orbitrap). The results showed that 96 metabolites contained in three kinds of Guizhou black teas were identified through chromatographic mass spectrometry analysis， including flavonoids， amino acids and their derivatives， nucleotides and their derivatives， and phenolic acids. According to the principal component analysis(PCA)， the three kinds of Guizhou black tea can be effectively distinguished， and it is concluded that there were significant differences between different varieties of black tea. Then， 23 kinds of differential metabolites among the three kinds of Guizhou black tea were screened out according to the FC value， P value and VIP value. Metabolic pathway analysis shows that its main metabolic pathway is the metabolism of flavonoids， which may be the reason for the differences in the taste of the three kinds of black teas. This study revealed the differential metabolites of the three kinds of Guizhou black teas from the perspective of metabolomics， and provided a reference for the identification of tea varieties.