Studies on the Removal of Zearalenone from Maize Using Magnetic Immobilized Horseradish Peroxidase
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(1.Harbin University, Harbin 150086;2.College of Food Science, Northeast Agricultural University, Harbin 150030;3.Jiusan Oils and Grains Industries Group Co., Ltd., Harbin 150090;4.High-skilled Talents (Model Worker) Innovation Studio of Qu Yanfeng, Harbin 150086)

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    Abstract:

    In this paper, magnetic nanoparticles Fe3O4-SiO2-HPG-COOH was prepared as immobilized carrier to covalently immobilize horseradish peroxidase (HRP), and applied to the degradation of zearalenone (ZEN). The immobilized HRP was characterized by Fourier transform infrared spectroscopy, X-ray diffraction and scanning electron microscopy, which verified that HRP was successfully immobilized on the carrier. The prepared nanoscale carrier Fe3O4-SiO2-HPG-COOH has uniform size and shape, good dispersion and strong magnetic properties. The saturation magnetization value was 25.80 emu/g, and the average particle size was 103.15 nm. Compared with free enzyme, the magnetically immobilized HRP indicated higher acid-base tolerance and thermal stability, the optimal pH value was increased from 6.5 to 7.0, the optimal temperature was increased from 30 ℃ to 35 ℃. Magnetic immobilized HRP was applied to ZEN degradation, and the relationship between solution pH, reaction temperature, hydrogen peroxide concentration and reaction time with ZEN degradation rate was investigated using single-factor experiments. The results showed that under the conditions of reaction temperature of 35 ℃, hydrogen peroxide concentration of solution 26 mmol/L, pH 7.0 and reaction time 480 min, the removal efficiency of ZEN was 68%. The immobilized HRP had good repeatability, and the relative enzyme activity remained above 79.85% after 5 cycles. This research shows that magnetic immobilized HRP has good degradation ability to ZEN.

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  • Received:July 09,2022
  • Online: August 17,2023
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